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Serum (thawed on ice) metabolites were extracted by adding 200 L of methanol at 80 C to 50 L of the serum sample, followed by vortexing for 5 min, incubation on ice for 10 min, and centrifugation at 4 C and 12,000 g for 15 min

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1 Of particular interest in this article is the function of GLP-1 as a hormone released by the gut during periods of eating that then stimulates the secretion of insulin

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