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They had ad libitum access to standard rodent chow and tap water throughout the study

Cysteine is described in nutritional literature as conditionally essential meaning it is not strictly essential (the body can produce it from methionine, another sulphur-containing amino acid) but the production pathway has limits, and dietary cysteine remains relevant

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Under oxidative or nitrosative stress, GSH covalently binds to exposed cysteine thiols on enzymes, protecting them from irreversible oxidation and modulating their activity

Many factors, including stress, diet, and aging, can reduce energy production at the cellular level