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DNA damage was quantified by scoring 100 randomly selected cells per slide, and damage percentage was calculated based on the proportion of cells showing comet formation

The primary antibodies used are as follows: P53 (Proteintech, 60283-2-Ig), pSer46-P53 (CST, 2521), FOXO4 (Proteintech, 21535-1-AP), Ki-67 (D3B5) Rabbit mAb (Alexa Fluor 647 Conjugate) (CST, 12075), P21 (CST, 14074), -H2AX (Abcam, ab2254)

Mitochondrial GSH (which makes up 1015% of total cellular GSH) is the primary defense line protecting mitochondrial DNA and the electron transport chain from radical-induced degradation

Ultrastructural alteration of pulmonary capillary endothelial glycocalyx during endotoxemia