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The resulting increases in superoxide (O 2 - ), hydrogen peroxide (H 2 O 2 ), hydroxyl radical, and free iron damage DNA, causing genetic mutations and initiating cancer progression

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To understand whether such variability in thiol content is related to the stage of nodule development at which the mutants are blocked or to metabolic changes in ineffective nodules, we also analyzed the root tissue and nodules of 1- and 2-week-old plants (Figure 6B)