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Peptide-antibody fusion production Anti-PCSK9 antibodies and peptide-antibody fusions were transiently expressed by co-transfection of the heavy and light chain expressing vectors into CHO cells using a polyethylenimine based method 47

The GST can be removed from the sample by re-chromatography on a glutathione column, and the protein of interest purified to homogeneity by other techniques such as gel filtration or ion exchange

As shown in Figure 7D, the wound area of all groups gradually decreased over a period of 15 days, and the RGD-GHK group exhibited the best wound-healing performance

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Be honest about how to read that: the doses in those studies were milligrams per kilogram, which is orders of magnitude above anything referenced in research protocols, and dose-limiting toxicology is designed to find effects