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Both I2 and HOI iodinate specific tyrosyl residues of the thyroglobulin within the colloid to form 3-monoiodityrosyl (MIT-yl) and 3,5-diiodityrosyl (DIT-yl) residuesintroducting iodine atoms at one or both locations ortho to the hydroxyls of tyrosine

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Measurement of Protein Content For the determination of protein content, the Lowry method [33], was employed