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Thus, sections were deparaffinized, rehydrated and subjected to antigen retrieval with citrate buffer, pH 6, containing 0.05% (vol/vol) Tween-20 (Sigma-Aldrich, Darmstadt, Germany), cooled in distilled water and permeabilized in 0.4% (vol/vol) Triton-X-100 in phosphate-buffered saline (PBS), pH 7.4, on ice for 30 min

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Five groups were used as controls: 2 groups were ovariectomized, treated with saline and sacrificed on the 15 th and 28 th day post-ovariectomy, 1 group was non-ovariectomized but treated with saline, and 2 non-ovariectomized groups were treated with NaBPC157 (10 g or 10 ng/kg b.w., i.p.) once daily for 28 days

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