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Discussion CRISPRCas9 is an RNA-guided gene-editing tool that offers several advantageous characteristics in comparison with the conventional methods (e.g., zinc finger nucleases and transcription activator-like effector nucleases)

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These findings indicate that the regulatory effects of distinct miRNAs on CUL5 vary across different tumor types and may be either positive or negative, underscoring the necessity of case-by-case investigation for specific miRNAs

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