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Abbreviations BAL: Bronchoalveolar lavage BALF: Bronchoalveolar lavage fluid COPD: chronic obstructive pulmonary disease CS: cigarette smoke CSE: cigarette smoke extract ELF: epithelial lining fluid GSH: glutathione References Pryor WA, Stone K: Oxidants in cigarette smoke

To validate this result, an alternative strategy to disrupt the pbgst gene was employed using a pbgst-ko plasmid with the hdhfr/yfcu positive-negative selectable marker (Figure 1E)

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