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In c) and f) the same proteins annotated in a), b), d) and e) are highlighted regardless their enrichment in this particular part of the experiment

Human PBMCs were plated in a 96-well plate in 100l and treated for 24 hours with cell stimulation cocktail (CSC), lipopolysaccharide (LPS) or R848

The column was then washed with 20 CV of DPBS/0.05% (v/v) sodium azide, followed by elution with 9 CV of 2 M MgCl 2 (Sigma-Aldrich)/20 mM Tris-HCl (Sigma-Aldrich) buffer (pH 7.4) at a flow rate of 2 mL/min

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