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These observations were corroborated by single-cell calcium imaging studies in slices of arcuate nuclei, demonstrating that GLP-1MK-801 inhibits the NMDA-induced intracellular calcium surge relative to GLP-1 alone (Extended Data Fig

This increase was weaker than that observed with fdx-2 or lias-1 knockdown in wild-type and slcr-46.1 mutant worms, indicating that partial loss of copper transport or binding capacity alone is insufficient to suppress cuproptosis