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Successively the cells were stimulated overnight with TNF- (100 ng/mL), washed with Dulbecco's PBS added with 2% BSA (Bovine Serum Albumine, fraction V, Sigma-Aldrich) and CaCl 2 -MgCl 2 0,7 mM (Sigma-Aldrich), and then incubated with mouse mAb anti-human VCAM1 (Sigma-Aldrich) 5 g/mL for 90 min at RT

The abdomen was cut open layer-by-layer cranially to create a 2-centimeter opening

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Generalized disease can involve all skeletal muscles, especially: Eye muscles: diplopia, blurred vision [6] Bulbar muscles: dysarthria, difficulty chewing and/or swallowing, dyspnea Proximal muscles: difficulty standing from a chair, climbing stairs, brushing hair [6] Respiratory muscles: dyspnea, respiratory failure Localized disease most commonly affects the eye muscles

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