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To aliquot safely: Work in a clean environment, ideally with minimal air movement Use sterile technique throughout Prepare labeled sterile vials or containers in advance Draw from the master vial and dispense into individual containers Refrigerate all aliquots immediately The trade-off is that aliquoting introduces more handling steps where contamination can occur

But NAC is more than just a glutathione precursor

The pause is what keeps the protocol delivering across multiple cycles

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You end up with a concentration you didn't intend, which throws off all subsequent dosing and measurements