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WT and ACLY KO cells were then transduced with a LentiCRISPR v2 vector with no guide RNA or containing a guide RNA targeting the first exon of ACSS2 or near the active site of CrAT: ACSS2 KO, DKO1, DKO2 (and KPACs): mACSS2sg2 - CGAGCTGCACCGGCGTTCTG DKO3: mACSS2sg6 - CTGCACCGGCGTTCTGTGG ACLY KO CrAT KO1, ACLY KO CrAT KO2: msgCRAT1F - CACCGTCCACAAGTGCAACTATGGG Following transduction, cells were treated with puromycin until the entirety of an un-transduced cell population died

Workouts can become tougher, and your recovery time may increase