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hiPSC culture for GRN knockdown and microglia differentiation The hiPSC lines used in this study were CRISPRi hiPSC 107 , an iPSC line with a constitutive CRISPRi machinery (dCas9-BFP-KRAB), and iTF-hiPSC 74 , an iPSC line expressing inducible CRISPRi machinery and inducibly expressing six transcription factors that enable the generation of microglia-like cells
Once a satisfactory length of LCx was exposed, the animal was heparinized (80 IU/kg), and a silastic vessel loop was passed to encircle the vessel
Naunyn-Schmiedeberg's Arch Pharmacol 399 , 1261312627 (2026)
They operate through largely distinct, yet potentially complementary, pathways
BMJ 339, b2535, 10.1136/bmj.b2535 (2009)
72 Hydrogen gas has been advocated as a novel and safe antioxidant strategy
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