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Lancet Diabetes Endocrinol 6(8):605617

Total DNA was extracted using 20 mM Tris pH 8.0, 5 mM EDTA, 400 mM NaCl, 1% SDS and 40 g/mL proteinase K, as previously described 110 , and used at 3 ng/mL for qPCR analysis of mitochondrial DNA (mtDNA) relative to genomic DNA (gDNA) as in ref

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