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The sections were then incubated with fluorescence-conjugated secondary antibodies (Alexa Fluor Cy3 and Alexa Fluor 488, 1:500, Beyotime, China)

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Consider the therapeutic target

Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity

doi: 10.1016/j.peptides.2020.170276 27 FukudaM

2124) retained the consistent clustering of early versus late mutations compared to germline, as well as the preferential grouping of ctDNA samples with specific mutations, with differences likely the result of enhanced phylogenetic signal when combining these data