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Ferric and ferrous ion assay Ferric and ferrous ion concentrations were assessed using the Ferric and Ferrous Ion Assay Kit (Beyotime, S1066)

If visible rubber fragments appear in the vial contents after a draw, the documented standard is to discard the vial regardless of remaining volume or days left in the window

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The glial glutamate transporter 1 (GLT1) is expressed by pancreatic beta-cells and prevents glutamate-induced beta-cell death

Slides were washed 3 times in PBST and incubated with the secondary antibody for DAB stain HRP horse anti-rabbit IgG Plus polymer KIT (ImmPRESS, MP-7801)

Measurement of Cytokine Secretion, Intracellular Protein Expression, and mRNA in Resting and Stimulated Peripheral Blood Mononuclear Cells