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All RNA samples had Agilent RNA Integrity Number values greater than 9.0

Again, these results are specific to experimental systems and do not imply established clinical efficacy

Subsequently, the PVDF membranes were socked in 5% skim milk containing 140 mmol/L NaCl, 20 mmol/L Tris-HCl (pH 7.5), and 0.1% Tween 20at room temperature for 60 min, and incubated with primary antibodies at 4C overnight: FXR mouse monoclonal antibody (72105S, CST, United States), TGR5 rabbitpolyclonal antibody (72,608, Abcam, United States), ZO-1 (ab216880, Abcam, United States), Occludin (ab 216,327, Abcam, United States), Claudin 2 (ab53032, Abcam, United States), P-P65 rabbit monoclonal antibody (3031S, CST, United States), P65 rabbit monoclonal antibody (8242S, CST, United States), -actin (Hua-an Biotech Inc., Hangzhou, China), and then incubated with horseradish peroxidase-conjugated secondary antibodies at room temperature for another 60 min

The Physician Perspective As a double board-certified internist and obesity-medicine specialist, I fully support advancing medical sciencebut only when its guided by rigorous research, regulatory oversight, and patient safety

Pregnant or breastfeeding individuals, children and adolescents, patients with active malignancy, complex autoimmune disease on biologics, or severe liver/kidney impairment are poor candidates for unapproved peptides with incomplete safety data

In chronic diseases, animals are administered half the dose