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Clear to pale blue is the correct appearance for viable GHK-Cu solution

The signal amplification mechanism was demonstrated by assessing the influences of various factors such as MB concentration, BSA concentration, solution pH and incubation temperature 14

Naliboff BD, Stephens AJ, Afari N et al: Widespread psychosocial difficulties in men and women with urologic chronic pelvic pain syndromes: Case-control findings from the multidisciplinary approach to the study of chronic pelvic pain research network

Therapeutic trial in the first three Asian cases of ethylmalonic encephalopathy: response to riboflavin

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2.5 Risk of bias assessment The risk of bias was assessed by two reviewers (LY, YY) respectively using the Cochrane Collaboration risk of bias tool for RCTs