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In fact, some different thiol-masking agents are routinely used to measure GSH and GSSG, such as alkylating agents (iodoacetic acid, NEM), 2-VP, 5-methyl methanethiosulfonate, monobromobimane [19]

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The study concluded that long-term antioxidant therapy with NAC helped prevent the development of pre-diabetes, dyslipidemia, and liver inflammation in obese rats, largely by reducing oxidative stress