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The fused macrocyclemRNA was subsequently reverse transcribed by RQ-RTase (Promega) for 1 h at 42 C and incubated with human recombinant Fc protein-immobilized Dynabeads protein G (Life Technologies) for 30 min at 4 C (negative selection against Fc-tag and beads)

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reports indicate induction of NO signaling and eNOS-linked vasodilatory effects, which may underlie faster perfusion and healing

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