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doi: 10.1016/j.bcp.2025.116797 171

For western blot analysis, cells were collected after 72 h of transfection and lysed in RIPA buffer with protease inhibitor (Roche) and phosSTOP (Roche)

They answer different quality questions: HPLC supports purity context, while MS or LC-MS/MS supports identity context

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While there are a few options, for GHK-Cu, the choice is usually straightforward