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Finally, 200 L of 20 mM ammonium bicarbonate were added and the protein lysates were proteolytically cleaved overnight at 37C with trypsin (2.5 L of 0.1 g/l trypsin, Promega)

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Indeed, activation of GLP-1R in different brain regions reduces food intake 6 and we clearly show that the OB is among these brain areas, with the demonstration that GLP-1-driven OB output activity also modulates food intake in a DIO model