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Phosphoenolpyruvate (6 mM), NADH (1 mM), pyruvate kinase (1 U/100 l), lactose dehydrogenase (1 U/100 l) and purified protein (1100 M) were diluted into the ATPase buffer (25 mM HEPES pH 7.5, 25 mM NaCl, 50 mM KCl, 4 mM MgCl 2 , 0.5 mM TCEP) and distributed into a 96-well plate to a final volume of 120 l

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