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For the preparation of mitochondrial subfractions, 1 mg of isolated mitochondria was suspended with 40 l T10E20 buffer (10 mM Tris-HCL, 1 mM EDTA, pH 7.6) and then incubated with digitonin (0.1 mg digitonin/mg of mitochondria protein) for 10 min on ice

PubMed: 12750433 KPV oral delivery via PepT1 PepT1 transporter uptake in inflamed intestinal tissue, murine IBD models, intracellular NF-B and MAP kinase suppression at nanomolar concentrations, PepT1-knockout loss-of-function proof: Kannengiesser K et al

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Recommended diluent: Bacteriostatic Water (BAC water) with 0.9% benzyl alcohol preservative

Hyperoside attenuates pregnancy loss through activating autophagy and suppressing inflammation in a rat model