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Krka, Slovenia)), we induced abdominal compartment syndrome by intraperitoneal insufflation of ordinary air controlled by a manual and digital manometer with a data logger connected to a computer (DD890, Dostmann Electronic GmbH, Germany) and maintained high abdominal pressure at 25 mmHg for 120 min before sacrifice, with a pressure measurement interval of 1 s

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Then, the NPT ensemble was further employed for 50 ns to equilibrate the system at 300 K and 1 atm, followed by a 10 ns production run for collecting equilibrated configurations at each 10 ps interval

Research Square Preprint2022 Jan