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[1] Cell Line:Cultured Sprague-Dawley rat Achilles tendon fibroblasts (passages 2-4) Concentration:2 g/mL Incubation Time:24 h Result:Increased F-actin (polymerized actin fibers, visualized as green fluorescence) levels notably relative to control cells with weak F-actin detection

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For its second application, the FDA set a PDUFA date of May 14, 2024, but this was pushed back by three months after Ascendis filed a major amendment to the application

Freezethaw cycles may: Affect molecular stability Increase aggregation risk in some formulations Reduce overall consistency of the solution Storage at 28C is typically preferred unless otherwise validated