In preliminary experiments, cyanide was first used in amounts (250 L of 1% NaCN solution (w/v)) equivalent to a literature value [14] for light-protected extractions in amber vials following the same procedures of the native SIDA method [7]
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Depletion of cellular GSH by using buthionine sulfoxamine (BSO), which inhibits glutamatecysteine ligase (the rate-limiting enzyme for GSH synthesis) is sufficient to induce ferroptosis due to impaired cellular antioxidant capacity 18
Fusion constructs between glutaredoxins and mutated fluorescent proteins furthermore provide valuable genetically encoded sensors for non-invasive redox measurements in vivo 9
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