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Preparation of Cell Free Extract Yeast cells grown in Sabouraud dextrose broth were harvested at 72 h of growth by centrifugation at 7,000x g washed with 0.5 M of potassium phosphate buffer, pH 7.5 containing phenylmethylsulfonyl fluoride (1 mM), EDTA (4 mM) and 2-mercaptoethanol (5 mM) and resuspended in the same buffer

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