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doi: 10.1016/j.phymed.2012.07.001

As a proxy for neuronal activity, neurons were exposed to anti-Syt1 lumenal primary antibody directly labeled with ATTO647N (1:200, 105 311AT1 clone 604.2 Synaptic Systems) in conditioned medium for 30 mins at 37 C

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But he remains optimistic about the promise of these drugs to reduce the risk of some cancers and believes the issue calls for further study

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The lack of ATP collapses the vesicular proton gradient, forcing stored catecholamines to leak out of the vesicles and flood the axoplasm/cytoplasm of the nerve terminal (Silver et al., 2001)