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The skull is exposed and after a craniectomy to expose the dura, an injury is produced by delivery of a fluid bolus, resulting from the release of a pendulum

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It is the standard reconstitution vehicle for lyophilised research peptides, including MOTS-c, SS-31, BPC-157, and GHK-Cu, as its bacteriostatic properties extend peptide solution stability significantly compared to plain sterile water