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The International Society of Sports Nutrition notes that resistance exercise and protein work together to support muscle protein synthesis, and that higher protein intakes may be useful during calorie restriction for lean mass retention in trained people

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The tissue homogenates were centrifuged for 12000 x g for 15 min and collected and diluted to 1 mg tissue protein per ml (1 mg/ml) in homogenization buffer (PBS, pH 7.4, containing a protease inhibitor cocktail) as the stock solution