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Samples were rinsed twice in thiol-free PBS (Life Technologies) prior to imaging with a Zeiss LSM700 confocal microscope (excitation at 405 nm and collection from 410500 nm)

After the vortex, the mixture was clarified by centrifugation at 1500 g for 10 min

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Long-acting agents such as semaglutide should generally be discontinued at least two months before attempting conception

Julien Dellinger, Institut de Gnomique Fonctionnelle de Lyon, cole Normale Suprieure de Lyon, CNRS UMR 5242, UCBL Lyon-1 Lyon F-69007, France

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