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To precipitate the salt and protein, 1 ml of methanol was added to the hair extracts, followed by vortexing for 30 seconds, and centrifuged at 4000 g for 5 min

RCTs with rigorous lifestyle control [e.g., standardized physical activity protocols] are needed to confirm causal relationships

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ACKNOWLEDGEMENTS Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study

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