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Given the pathological role of oxidative stress generation in MCP-1 induction and apoptotic cell death in tubular cells, hyperglycemia-elicited ROS generation might be a therapeutic target of tofogliflozin (157, 158)

BFP+GFP-cells were collected in a Falcon tube containing 100% FCS, centrifuged, and rapidly transferred to T25 flasks containing normal culture medium

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