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Viral infection For lentiviral production, HEK293T cells were co-transfected with 4 g 8.91, 1 g pVSVG, 5 g of lentivector (shRNA targeting SHMT2 : CCAGAATTTGTAGCTGAGATT, and ATF4 : GCGAGTGTAAGGAGCTAGAAA, obtained from RNA Technology platform and Gene manipulating core, Academia Sinica) by using 30 L of Turbofect (Thermo, R0533) in 1 mL of Opti-MEM (Gibco, 31985070) according to the manufacturers instructions
Further, the assay is affected by pH, salt concentration, temperature, kind of buffer, and contaminants that may be present in the cuvette
Ihre Vorteile: Verfeinerte Hautstruktur und mehr Spannkraft
Soerens, A
*Disclaimer:These statements have not been evaluated by the Food and Drug Administration
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