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The tissue was then blotted dry and weighed followed by homogenization in 200 l buffer (0.05 M potassium phosphate and 0.1 mM EDTA, pH 7.8) and centrifuged at 15,000 xg for 30 min at 4C

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Too much pressure pushes the liquid laterally into less absorptive tissue

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That issue has since subsided after Eli Lilly and Nordisk raced to ramp up manufacturing capacity for the drugs

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