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The eluted proteins were dialyzed against buffer A using an Amicon Ultra-15 device with a molecular cutoff of 10 kDa (Millipore, UFC901008) and concentrated to 0.5 ml
Downregulated GPX4 expression increases the susceptibility of cells to ferroptosis, whereas upregulated GPX4 expression has the opposite impact [24]
5.3 Antidepressant and anxiolytic effects Elevated levels of systemic inflammation, neuroinflammation, and hyperactivity of the hypothalamicpituitaryadrenal (HPA) axis have been identified as key factors contributing to the pathogenesis of neuropsychiatric disorders, including depression and anxiety (7074)
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It has also been reported that glutathione plays a role in the redox pathway of mitochondrial dynamics regulation in axons (Mariet et al
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