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They proposed that weak acids could catalyze nonreducing amination reactions and enhance derivatization reactivity and ionization efficiency if traditional organic acid matrices were combined with acid-catalyzed reactive matrices

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Next, the proteins were purified by gel filtration in a Superdex 200 column (Amersham Biosciences Inc., Piscataway, NJ, USA) equilibrated in 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl