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n = 5, both males and females), mice were killed by cervical dislocation, and the glabrous skin from one hind paw with the tibial nerve attached was dissected and placed in a custom-made two-compartment Teflon recording chamber with the corium side down

However, many questions remain about how exactly the drugs work, who should be taking them, how long they should be taken for, as well as what side effects users should be wary of

A blinded assessment of the histopathology of the liver and kidney of treated animals was conducted 6 weeks after treatment (Figure S5 and S6)

Permeabilization was then performed using PBS containing 0.5% Triton X-100 (108,603, Merck, Darmstadt, Germany) for 1 h at room temperature, followed by washing twice for 5 min in PBS/BSA