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A standard curve was plotted prior to each analytical run

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Ensuring the solution is at room temperature before injecting also decreases the likelihood of irritation

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The GTT indicated significantly lower blood glucose AUC in Donor+Cas9 treated mice compared to Saline and Donor groups at 8, 16, and 24 weeks post-injection, with levels comparable to non-treated mice fed with normal fat diet (Normal Diet) at matching time points