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Quantification of mRNA was performed by using primers that span over two adjacent exons, quantified using the comparative standard curve method and normalized to hypoxanthine phosphoribosyltransferase ( Hprt ), a housekeeping gene

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Interestingly, FGF21, SREBP-1c, and FAS mRNA in the liver tissues of the model group of mice and protein expression was increased compared with the control group, while liver kinase B1 (LKB1) mRNA expression was reduced