10.3390/antiox6020024 199 MartinaV.BrunoG.ZumpanoE.OrigliaC.QuarantaL.PescarmonaG
They act as antioxidants, which means they help fight off damage from pollution, stress, and unhealthy foods
GHK-Cu (50mg): A copper-binding tripeptide used in research involving peptidemetal interactions and copper-associated signalling pathways
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Cell culture Cell lines were maintained in RPMI 1640 (Thermo Fisher, 11875119) with 5% FBS (Sigma-Aldrich, SH30396.03) and 1% penicillin and streptomycin (Thermo Fisher, 15070063)
On day 2, slices were washed 35 min in PBS 0.01 M at RT 4 mL/well and incubated in donkey anti-guinea pig-AF647 (1:500, Jackson ImmunoResearch) diluted in [PBS 0.01 M + 0.3% Triton]