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Immunoblotting Protein samples in 1x NuPage LDS sample buffer, 25 mM DTT (or 50 mM TCEP pH 7.0) were loaded on to SDS PAGE gels (Invitrogen, XP04205) and transferred to nitrocellulose membranes

Tirzepatide, developed by Eli Lilly, is the worlds first dual GLP-1R/GIPR agonist and has shown superior weight loss effects compared to semaglutide

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Collagen IV stimulation

Mechanistically, syndecans induce protein kinase C-alpha (PKC)-dependent phosphorylation of a conserved serine residue in the cytoplasmic domain of TRPC channels 3

The mechanism: repeated trauma to the same muscle belly triggers localized inflammatory adipogenesis, where mesenchymal stem cells differentiate into adipocytes in response to tissue injury signals