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Protein concentration was determined using a BCA kit (Thermo Scientific), after which samples were diluted to 1 mg ml 1 in 5% SDS and 50 mM TEAB

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When exposed to these toxins, glutathione is consumed along with them and needs to regenerate

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Many practices make the mistake of assuming the patient is without crisis simply because they arent in severe pain or hospitalized

This stimulation doesn't just replace lost collagen