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Acute and chronic consumption of sulforaphane consumption ameliorated anxiety and depressive-like behaviours in ICR and C57BL/6 mouse models (assessed using the novelty suppressed feeding test, open field and tail suspension tests) (Wu et al., 2016

After excess BHZ was rinsed away with sterile PBS (3 times, 15 min each), the samples were fixed in 3.7% formaldehyde for 1 h before immunofluorescence staining

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Reactive oxygen species can attack biomembranes, enzymes, proteins, and nucleic acids (Venditti et al., 2013)