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After washing the cells with saline (9 g L 1 NaCl), the cells were pelleted again by centrifugation (10 min, 12,000 g , 4C) and then all samples were frozen at 20C until analysis via LC-MS

While WT albumin showed slightly weaker binding to FcRn at pH 5.5 upon enzymatic cleavage, L585-cleaved QMP bound to the receptor as strongly as full-length QMP (Fig

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