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This is considered to be the rate limiting step because it is dependent on the bioavailability of cysteine and the activity of GCL, the latter of which is modified by competitive inhibition by reduced glutathione (GSH) [3134]

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While this may facilitate the entry of immune cells to fight infectious agents, it also provides an avenue for luminal contents including commensal microbes, pathogens, and dietary components, to bypass the intestinal epithelium and enter systemic circulation