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In between each treatment, the booster helps maintain a steady level of Glutathione for optimal results

The GSH/GSSG ratio is widely recognized as a sensitive indicator of oxidative stress (Kern & Jones, 2006

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HSA, was from Sigma or purified from serum as described below

Notably, optimal callus growth occurred at the same NM concentrations that yielded maximum pigment accumulation, corroborating with earlier study 69

Oxidative stress biomarkers were immediately estimated from the supernatant, or samples were stored at 80 C for further analysis within one month